Principles and Practice of Bioanalysis, Second Edition

Richard F. Venn
2/25/2008
Explores the current practice of bioanalysis in the pharmaceutical industry and in drug metabolism and pharmacokinetics laboratories Demonstrates how a thorough understanding of physicochemical properties of drugs and metabolites facilitates bioanalysis Focuses on commonly used techniques such as protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) Covers reversed-phase, ion-pair, hydrophilic interaction liquid chromatography (HILIC), and chiral HPLC Examines the pros and cons of immunoassay, their practical aspects, and when to consider using them, highlighted by practical examples from the pharmaceutical industry Discusses commercially available instruments that illustrate various approaches to automation

In the seven years since the publication ofPrinciples and Practice of Bioanalysisbioanalytical methods have remained the same, but their usage patterns have changed. This second edition of a bestseller provides an updated guide to the techniques used in developing and running ultra-trace analyses for drugs, metabolites, and other substances.

Extensively revised, this volume reflects the dynamic growth in methodologies that has occurred over the past five years. Each chapter has been overhauled or re-written to reflect modern practice. Experts in the field offer a logical path through the problems of small molecule bioanalysis, enabling you to choose the appropriate methods of analysis across a number of fields, including the agrochemical and pharmaceutical industries. They cover a wide range of analytical methods, including mass spectrometry, liquid chromatography, immunoassay, NMR, and gas chromatography.

Presenting the information in an informal and highly accessible style, the book covers the theory and practice of bioanalysis, enabling you to gain maximum benefit and to optimize analytical methods. With practical advice and guidance, it delineates how to develop robust analytical methods for a range of compounds, both natural and synthetic. Using these insights, you can more easily recognize problems and quickly solve them.

Table of Contents
Physicochemical Properties of Drugs and Metabolites and Their Impact on Bioanalysis,H. Wiltshire and A. Nedderman
Sample Preparation,C.A. James
Basic HPLC Theory and Practice,A. Gray and P. Lawrence
HPLC Optimisation,D. Bakes
HPLC Detectors,K.C. Saunders and R.F. Venn
Gas Chromatography: What It Is and How We Use It, P. Andrew and M.V. Doig
Thin-Layer Chromatography,H. Wiltshire and A. Nedderman
Immunoassay Techniques,R.F. Venn
Automation of Sample Preparation,K.C. Saunders and C.A. James
Fundamental Aspects of Mass Spectrometry,M.V. Doig and D.M. Higton
Quantitative LC 朚S,D.M. Higton and M.V. Doig
Mass Spectrometric Identification of Metabolites,G. Dear
Nuclear Magnetic Resonance In Drug Metabolism,P. Gilbert
Metabolite Isolation,G. Dear, A. Nedderman, and H. Wiltshire
Strategy for the Development of Quantitative Bioanalytical Procedures,D. BakesIndex


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