Richard B. Thompson 12/16/2005 Search this book Provides an up-to-date account of fluorescence-based sensors with an accessible approach suitable for newcomers as well as the more advanced researcher Illustrates the high intrinsic sensitivity, flexibility, and ability of fluorescent sensors to remotely image chemical analytes Highlights new recognition and transduction approaches, new technologies, and several practical applications including lab-on-a-chip devices Discusses metal-enhanced fluorescence, phosphorescent labels, fluorescent probes selective for anions, and a unique technology for fluid-handling on the nanoscale Includes abundant illustrations and color figures
Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics.Fluorescence Sensors and Biosensorsemphasizes the most recent developments and emerging technologies with the broadest impacts.
The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform. for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations.
The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields,Fluorescence Sensors and Biosensorsprovides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.
Search this book Table of Contents Introduction; T. Vo-Dinh Prospects for theDe NovoDesign of NucleicAcid Biosensors;L. Yang and A.D. Ellington Biosensors Based on Periplasmic Binding Proteins;B.V. Sharma, S.S. Shrestha, S.K. Deo, and S. Daunert Molecular Beacon DNA Probes Based on Fluorescence Biosensing;G. Yao, M.S. John, and W. Tan Fluorescence Resonance Energy Transfer-Based Sensors for Bioanalysis;G. Blagoi, N. Rosenzweig, and Z. Rosenzweig Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects;R.B. Thompson, R.A. Bozym, M.L. Cramer, A.V. Stoddard, N.K. Westerberg, and C.A. Fierke Metal-Enhanced Fluorescence Sensing;C.D. Geddes, K. Aslan, I. Gryczynski, J. Malicka, and J.R. Lakowicz Subpicomolar Assays of Antibodies and DNA Using Phosphorescence Labels;A.P. Savitsky Development of Fluorescent Dipyrrolylquinoxaline-Based Anion Sensors;P. Anzenbacher, Jr. and K. Jurs韐ov?/i> Lab-on-a-Chip and Fluorescence Sensing on the Microscale;H.N. Chang, A.V. Hatch, K.R. Hawkins, and P. Yager The Array Biosensors;F.S. Ligler and C.R. Taitt Planar Waveguide Biosensors for Point-of-Care Clinical and Molecular Diagnostics;J.N. Herron, H.-K. Wang, L. Tan, S.Z. Brown, A.H. Terry, J.D. Durtschi, E.M. Simon, M.E. Astill, R.S. Smith, and D.A. Christensen Fluorescence-Based Sensors for Bioprocess Monitoring;L. Tolosa, Y. Kostov, and G. Rao Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist;R.B. Thompson, C.J. Frederickson, C.A. Fierke, N.M. Westerberg, R.A. Bozym, M.L. Cramer, and M. HershfinkelIndex
