紫外吸收光谱测定蒽醌试样中蒽醌的含量
<p class="MsoNormal" style="LINE-HEIGHT: 150%"><strong><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">实验目的</span><span lang="EN-US">:<p /></span></strong></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">1.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">了解</span><span lang="EN-US">UV-4100</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">型紫外</span><span lang="EN-US">-</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">可见</span><span lang="EN-US">-</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">近红外分光光度计的基本构造、基本</span></p><p class="MsoNormal" style="LINE-HEIGHT: 150%"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">工作原理和基本操作步骤；</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">2.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">掌握用紫外吸收光谱测定蒽醌试样中蒽醌的含量。</span></p><p class="MsoNormal" style="LINE-HEIGHT: 150%"><b><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">实验原理</span></b><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">：</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">蒽醌在波长为</span><span lang="EN-US">251 nm</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">处有一强峰和</span><span lang="EN-US">323 nm</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">处有一中强峰，但是在</span><span lang="EN-US">251</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">处有邻苯二甲酸酐的吸收峰干扰，所以选择在</span><span lang="EN-US">323</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">处测定蒽醌试样中蒽醌的含量。</span></p><p class="MsoNormal" style="LINE-HEIGHT: 150%"><b><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">实验步骤：</span><span lang="EN-US"><p /></span></b></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">1.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">蒽醌系列标准溶液的配制</span><span lang="EN-US"><span style="mso-spacerun: yes"> </span></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">在</span><span lang="EN-US">5</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">只</span><span lang="EN-US">10 mL</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">的比色管中分别加入</span><span lang="EN-US">2.00</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">，</span><span lang="EN-US">4.00</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">，</span><span lang="EN-US">6.00</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">，</span><span lang="EN-US">8.00</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">，</span><span lang="EN-US">10.00 mL</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">蒽醌标准溶液（</span><chmetcnv style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x" tabindex="0" tcsc="0" numbertype="1" negative="False" hasspace="True" sourcevalue=".04" unitname="g"><span lang="EN-US">0.0400 g</span></chmetcnv><span lang="EN-US">.L<sup>-1</sup></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">），然后用甲醇稀释到刻度，摇匀备用。</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">2.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">称取</span><chmetcnv style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x" tabindex="0" tcsc="0" numbertype="1" negative="False" hasspace="True" sourcevalue=".1" unitname="g"><span lang="EN-US">0.1000 g</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">蒽醌试样于小烧杯中，用甲醇溶解后，转移至</span><span lang="EN-US">50 mL</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">容量瓶中，以甲醇稀释至刻度，摇匀备用。</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">3.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">用</span><chmetcnv style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x" tabindex="0" tcsc="0" numbertype="1" negative="False" hasspace="True" sourcevalue="1" unitname="cm"><span lang="EN-US">1 cm</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">石英吸收池，以甲醇做参比溶液，在</span><span lang="EN-US">200~400 nm </span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">波长范围内测定一份蒽醌标准溶液的紫外吸收光谱。</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">4.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">在选定的波长，以甲醇为参比溶液，测定蒽醌系列标准溶液以及蒽醌试样的吸光度。以蒽醌标准溶液的吸光度为纵坐标，浓度为横坐标，绘制标准曲线，根据蒽醌试液的吸光度，在标准曲线上查得其对应的浓度，并根据试样的配制情况，计算蒽醌试样中蒽醌的含量。</span></p><p class="MsoNormal" style="LINE-HEIGHT: 150%"><b><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">数据处理：</span><span lang="EN-US"><p /></span></b></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">1.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">原始数据</span></p><table class="MsoNormalTable" style="BORDER-RIGHT: medium none; BORDER-TOP: medium none; MARGIN-LEFT: 6.2pt; BORDER-LEFT: medium none; BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-border-alt: solid windowtext .5pt; mso-border-insideh: .5pt solid windowtext; mso-border-insidev: .5pt solid windowtext" cellspacing="0" cellpadding="0" border="1"><tbody><tr style="HEIGHT: 21.3pt; mso-yfti-irow: 0"><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 1pt solid; WIDTH: 107.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt" valign="top" width="143"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">浓度（</span><span lang="EN-US">10</span><chmetcnv style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x" tabindex="0" tcsc="0" numbertype="1" negative="True" hasspace="False" sourcevalue="3" unitname="g"><sup><span lang="EN-US">-3</span></sup><span lang="EN-US">g</span></chmetcnv><span lang="EN-US">.L<sup>-1</sup></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">）</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 55.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="73"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">8</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 35.4pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="47"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">16</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 63.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="85"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">24</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 43.75pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="58"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">32</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 54pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="72"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">40</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 51.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 21.3pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="69"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><i><span lang="EN-US">x</span></i></p></td></tr><tr style="HEIGHT: 27.8pt; mso-yfti-irow: 1; mso-yfti-lastrow: yes"><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 1pt solid; WIDTH: 107.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="143"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">吸光度</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 55.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="73"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">0.255</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 35.4pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="47"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">0.522</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 63.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="85"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">0.876</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 43.75pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="58"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">1.122</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 54pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="72"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">1.562</span></p></td><td style="BORDER-RIGHT: windowtext 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 51.55pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 1pt solid; HEIGHT: 27.8pt; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="69"><p class="MsoNormal" style="LINE-HEIGHT: 150%; TEXT-ALIGN: center" align="center"><span lang="EN-US">0.874</span></p></td></tr></tbody></table><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">2.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">绘制出来的标准曲线</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">3.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">计算蒽醌试液浓度的大小。</span></p><p class="MsoNormal" style="LINE-HEIGHT: 150%"><b><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">思考题：</span><span lang="EN-US"><p /></span></b></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">1.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">为什么选用</span><span lang="EN-US">323 nm</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">而不选用</span><span lang="EN-US">251 nm</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">波长作为蒽醌定量分析的测定波长？</span></p><p class="MsoNormal" style="TEXT-INDENT: 21pt; LINE-HEIGHT: 150%; mso-char-indent-count: 2.0"><span lang="EN-US">2.</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: 'Times New Roman'; mso-ascii-font-family: 'Times New Roman'">本实验为什么用甲醇作参比溶液？</span></p> 紫外是不是不能把蒽醌类中的大黄酸、大黄素、大黄酚完全分开？哪两个不能分开呢页:
[1]
