Proteomic Tools for the Isolation and Analysis of ex vivo Protein Complexes
1. Protein complex affinity purification and mass spectrometry (AP-MS)
background
2. Detail each step of the AP-MS procedure (techniques that we use)
3. Proteomic developments that address AP-MS limitations
• Contamination / non-specific interactions
• Stoichiometry
• Multiplicity
• Transient interactions
• Dynamics
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