[新加坡招聘博士后]细胞学方向

[新加坡招聘博士后]细胞学方向
发信站: 水木社区 (Fri Feb 29 16:28:39 2008), 站内

我的一位朋友，在新加坡国立大学做assistant professor，研究的方向相当有潜力。现欲
招“细胞中分子的动态结构与纳米分辨率的三维成像技术”方向的博士后。以下为他的个人
主页地址（打开速度较慢请耐心等待）：http://www.dbs.nus.edu.sg/staff/hewz.htm

代为发帖，有意者请直接联系，莫联系本人。

Two postdoctoral positions in cellular electron tomography in Singapore

Two postdoctoral positions are available from April 2008 in Dr. Wanzhong He’s
Cellular Electron Tomography and Cryo-EM Lab at Dept. of Biological Sciences,
National University of Singapore. This research lab is focused on nano-imaging
of molecular architectures and cellular dynamic events in several in vivo
engineered animal model systems with the combination of several advanced
technologies that have been established in the lab, e.g.:  high pressure
freezing (HPF), freeze-substitution fixation (FSF),  frozen-hydrated thin
sectioning, our newly developed HPF/FSF-based nanogold-enlarging technique (He
et al., JSB, 2007, 160(1):103-113; He et al, manuscript submitted to Nature,
considered as a research article, under revision),  high resolution
immunolabelling, and cellular electron tomography (He et al., Science 2003,
v302,109-113). Although it is possible to identify a few large proteins or
complex in cells at a few nanometer resolution with HPF/FSF or frozen-hydrated
samples, however, there are still questionable with inevitable artifacts caused
by dehydration, mechanical sectioning distortion, high accumulated electron
dose damage, subjective tomographic averaging methods etc. The majority of the
proteins in the cellular environment are very small and impossible to be
identified directly from the 2-5nm unreliable “densities” (or “molecular
signatures”).  How to identify/map out those small proteins in the cells from
tomograms with 2-5nm ultrastructural background would be a quite challenging
but a very important direction for cellular electron tomography in the future.
Our lab will continue to develop new nano-technologies to identify:  What are
these small proteins? Where are they distributed and perform their functions?
When do they interact with other proteins?

Position I:  is funded by A*STAR (Agency for Science, Technology and Research),
Singapore. We will use genetic engineered medakafish model together with above
mentioned nano-imaging technologies to trace the stem cells and their
differentiation from embryo to adult, from micrometer to nanometer resolution
in 3D. It will be closely collaborated with a stem cell and medakafish genetics
experts in the same department.

Position II: is funded by MOE (Ministry of Education), Singapore. We will
focused on tracing several receptor-mediated endocytosis processes in rat small
intestinal epithelial cells with 1.4nm nanogold particles labeled ligands, high
resolution labeling technologies and cellular electron tomography.

We have ordered two cryo-electron microscopes: a FEI T12 with 4kx4k Gatan
ultrascan UHS CCD; a state-of-the-art FEI Titan Krios 300kV FEG cryo-electron
microscope with 4kx4k Gatan ultrascan US4000 UHS CCD and Gatan Tridiem energy
filter imaging system(one of the best in the world); and a vitrobot. Currently,
my lab equipped with a high pressure freezing machine, 2 freeze-substitution
machine, 2 cryo-ultramicrotome, a CM-10 100kV electron microscope and a
JEOL-2010F cryo-electron microscope, and a lot of other facilities, like JEOL
carbon/gold coating system, plasama cleaner, glow discharging device, JEOL SEM,
dark room, workstations, light microscopes, biosafety cabinet class 2,
autoclave, etc.

Qualifications include a Ph.D. degree and strong background in: 1) Cell Biology,
Genetics and Developmental Biology, Biochemistry, Structural Biology etc.; 2)
Or in Material sciences, Physics or Chemistry with strong TEM expertise will be
considered.  Experiences in transmission electron microscopy and computer
skills will be a plus. Salary will be comparable or higher than  NIH standard
(S$500-600k/yr), with additional house allowance (S$6000/yr) and medical
insurance. Interested candidates should send a CV including areas of expertise
and interest, publications list, and names and contact information for three
references to Dr. Wanzhong He at dbshw@nus.edu.sg

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Dr. Wanzhong He (Assistant Professor)
Department of Biological Sciences
National University of Singapore
14 Science Drive 4, Singapore 117543

Tel: +65 6516 7378   Fax: +65 6779 2486
Email: dbshw@nus.edu.sg
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